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Chemical Test Sporal "A" 1 pc.
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Biological indicator of control of the steam-formaldehyde sterilization process DS-100
STOCK AND PROPERTY:
The indicator is shaped in the form of a bubble tape saturated with a bacillus stearothemophilus ATCC 7953 (for Form) and Bacillus
Atrophaeus ATCC 9372 in one special paper packaging that protects against contamination
contains not less than 1 x lOs of units capable of transition into vegetative form (CFU).
Differences contained in indicators exposed to saturated water steam under pressure at a temperature of 121° C (I arm.) in time
5 minutes spend 100%, disputes under the same conditions within 15 minutes undergo total inactivation
0 % of growth.The value D for a temperature of 121°C is not less than 1.5 minutes (this is the decimal reduction rate, i.e. time)
expressed in minutes necessary for a reduction in the test microorganisms of the order size at a given temperature).
For formaldehyde sterilization, the test should be exposed to 1-2% formalin within 60-90 minutes.
WAYS OF USE:
• Test indicators to be placed in at least two of the largest representative packages for a particular charging and to be placed in
the most difficult places for the sterilizer.
• Implementation of a sterilization program
• After the sterilization process is completed, remove the test indicators from the load and make the seating within 5 hours after exposure.
If the indicators cannot be incubated in the given, the time should be placed at room temperature up to 24 hours,
The food control of microorganisms is an indicator not subject to the sterilization process.
• Tests, after use and determined tests sterilize in the autoclave or burn
For Formaldehyde or E0 sterilization, the biological test of each HODOLVVA plant is carried out
• Keeping aseptic conditions to remove the bubble stripes from paper-fold bags and put each in a separate prob
bacteriological from the substrate of the TSB (Soy Broth mode) — the kazeino-soy bulion — the bag can be cut anywhere.
It should be noted that the bubble bar is completely immersed in the bubble
• Incubate, 5 days at a temperature of 55 +/- 2 °C
In probes containing bibuella stripes of the sterilized indicators, no increase should occur.Appearance
the growth indicates an irregular process of sterilization.
• Tests should be observed daily to determine possible growth.In case of ineffective sterilization in 90%
the increase will occur within 48 hours.However, it should always be observed for 5 days.
In the probes containing the indicator not sterilized, the increase should occur within 48 hours.
NORMS
- Products comply with EN 866 for biological control and ISO 11138
PRODUCER
SPS Medical NY USA, Henrietta Road
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Biological indicator for the control of the steam-formaldehyde sterilization process DS-100
COMPOSITION AND PROPERTIES:
The indicator has the form of a strip paper saturated with a suspension of spores of the type Bacillus stearothemophilus ATCC 7953 (for Form) and Bacillus
Atrophaeus ATCC 9372 in one special paper package to protect against contamination Each indicator
contains not less than 1 x lOs units capable of transforming into vegetative forms (CFU).
Spores contained in indicators exposed to saturated steam under pressure at a temperature of 121° C (1st arm.) survive 100% for
5 minutes, spores exposed to the same conditions for 15 minutes are completely inactivated
(0% growth). D value for temperature 121° C is not less than 1.5 minutes (this is the decimal reduction coefficient, i.e. the time expressed in minutes necessary for the test microorganisms to be reduced by an order of magnitude at a given temperature).
In the case of formaldehyde sterilization, the test should be exposed to 1-2% formalin for 60-90 minutes.
HOW TO USE:
• Place the test indicators in at least two of the largest representative packages for a given load and place them in
places that are most difficult to access for the sterilizing agent.
• Carry out the established sterilization program
• After completing the sterilization process, remove the test indicators from the load and inoculate within 5 hours after exposure.
If the indicators cannot be incubated within the specified time, they should be placed at room temperature for up to 24 hours,
• Food control of microorganisms is an indicator that has not been subjected to the sterilization process.
• Sterilize tests after use and expired tests in an autoclave or incinerate
• For Formaldehyde or E0 sterilization, a biological test is performed on each HODOLVVA
• Maintaining aseptic conditions, remove the strips of tissue paper from the paper and foil bags and place each in a separate bacteriological test tube with TSB (Soy Broth mode) the bag can be cut anywhere.
Make sure that the strip of tissue paper is completely immersed in the broth
• Incubate for 5 days at 55 +/- 2°C
Growth should not occur in test tubes containing paper strips from sterilized indicators. The appearance of
growth indicates an incorrect sterilization process.
• The tubes should be observed daily to detect any growth. In the case of ineffective sterilization, in 90%
of cases, growth will occur within 48 hours. However, you should always observe the tubes for 5 days.
• In a tube containing an unsterilized indicator, growth should occur within 48 hours.
STANDARDS
- Products meet the EN 866 standard for biological control and ISO 11138
MANUFACTURER
SPS Medical NY USA, Henrietta Road
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